58 Report OF DEPARTMENT OF BACTERIOLOGY OF THE 
TABLE XI.— SoLuBLE NITROGEN IN CHLOROFORMED SKIM-MILK.* 


PERCENTAGE OF CHLOROFORM, 





INTERVAL. — 
220 Dou 10.0 20.0 30.0 
Soluble nitrogen expressed] in percent/age of total] nitrogen. 
MGA SI! x iekiet tite eg eta cine Mes sens 10.59 9.63 9.85 9.97 13.33 
9.00 11.19 12.49 11.85 8.08 
4 MySnag Ws RENO Ria Ae tom 16.09 13.47 13.87 13.76 el Wa 33 
' 10.94 ee edi 15.90 18.42 15.85 
20 Re Cols Ae nO ee Se he 22. 08 22.82 22.08 PREM | 24.32 
162 27 21.99 222 20.26 21-12 
EDM VS eRe ee ore ee kee tate ceete S171 31.94 30.29 STne oatce 
21.65 34.59 32.00 28.09 32.76 
192 awe rise tye pF ee aa 42.18 38.83 34.79 35.82 36.40 
35.41 39.54 37.76 38.88 35.01 
DaOeayereearauluieek tee ys 51.81 47.52 42.19 44.47 45.40 
43.89 52.54 46.97 47.04 42.92 
Bacteria per cubic centime|ter found i/n these mil|ks. 
MEGA SOs, Chertiake Ris eee tad deavaiote 34 60 25 22 18 
De 33 27 28.4 lest 
DTA Se atest chan valeroU! aie Me eee 39 30 25 22 31 
45 41 i 26 31 29 
DAY Sisko s wus betes Wiener Sohn en rere Fe, Bac 11 6 8 
Ra RPA tilts tea ees * 11 5 3 
NO DEANS eee CE el ih ool ely pee 14 7 6 aceon ee 
13. [yc slave dene Pik clggieinte a eet Divs) 0a. r 
2 8 6 6 2 
BAO GAayss sai heehee taewea hay eats 10 8 8 12 6 


* Chemical determinations by Messrs. Le Clerc, Patten and Hart, and bacteriological by 
Mr. Rogers. 
The large percentages of chloroform promptly reduced the 
bacteria to numbers which represented only the most resistant 
spores in the original milk. 
A comparison of the results here given in case of 2.5 per ct. 
of chloroform with those obtained under similar conditions as 
given in Table X shows that in the two experiments the diges- 
tion progressed at approximately the same rate under similar 
conditions. 
In this table the analyses of the pairs of bottles are given 
each time in the same order and it will be noticed that there are 
distinct differences in the rate of digestion in the different bottles. 
Thus the soluble nitrogen in one of the bottles containing 2.5 
per ct. of chloroform is high during the entire experiment, while, 
at the same time, in its duplicate the percentage of soluble nitrogen 
was among the lowest in the entire series. 
While the differences are not marked, there is indication of a 
slight retarding action with the increasing percentages of chlo-_ 
roform, but this is by no means so well marked as with the range 
from 0.7 to 2.5 per ct. 
-_— =. ee 
