t 
62 REpoRT OF DEPARTMENT OF BACTERIOLOGY OF THE 
Action upon milk proteids—— The chloroform above that required 
to saturate the fat and the milk-serum carries down casein in 
quantities roughly proportional to the excess of chloroform pres- 
ent. This combination is readily observed as a heavy, white, 
opaque layer. The casein does not appear to be changed by this 
solution in the chloroform since it can be separated from the 
chloroform by vigorous shaking with water and when thus sepa- 
rated responds to reagents as does ordinary milk-casein. 
General.— Chloroform is a fairly satisfactory agent for repress- 
ing germ life in connection with the study of milk enzyms. 
Quantitative studies of the action of such enzyms should receive 
a’correction of at least 10 per ct. where 2.5 per ct. of chloroform 
or more is used. 
LITERATURE. 
CHLOROFORM AS RELATED TO THE STUDY OF ENZYMS. 
Babcock, S. M., & Russell, H. L. (1) Unorganized ferments of milk: A new 
factor in the ripening of cheese. Wis. Agr. Exp. Sta. Rept. 14: 161-193. 
1897. Similar article with same title. Cent. Bakt. u. Par., Il Abt., 3: 
615-620. 1897. (2) Tolerance of certain milk bacteria toward ether. 
Wis. Agr. Exp. Sta. Rept. 14: 211-215. 1897. ——————-. —————— & 
Vivian, A., (3) The antiseptic value of certain chemicals in milk. Wis. 
Agr. Expt. Sta. Rept., 15: 98-103. 1808. 
Bertels, A. (1) Einflus des Chloroforms auf die Pepsinverdauung. Virchow’s 
Arch. path. Anat. u. Physiol., 130: 497-511. Abstract in Chem. Centbl., 
642°C 1) 15867 1803: 
Buxton, D. W. (1) Empiricism or science: Anaesthetics 1847-1897. Lancet 
[London] 2: 1369-1376. 1897. Abstract in Exp. Sta. Rec., 9: 6095. 
1808. 
Carini. (1) Ueber Methoden schneller Bakterienbefreiung der frisch abge- 
nomenen Kuhpockenlymphe. Riv. Igiene e Sanita pub. [Torino], 16: 
1905. Abstract in Cent. Bakt. u. Par., I Abt., Ref.,.36: 47-50. 1905. 
Croix, De la. (1) Arch. exp. Path., 13: 250. Cited by Kirchner. 
Cushny, Arthur. (1) Ueber Chloroform und Aethernakose. Ztschr. Biol., 
28: 365-404. 1891. Good bibliography. 
Dubs. (1) Einfluss des Chloroforms auf die kunstliche Pepsinverdauung. 
Virchow’s Arch., 134: 519-540; abs. in Chem. Centbl., 65 (1): 177. 1894. 
Eberhard. (1) Chloroformbildung aus Milchsaure. Apoth. Zeit., 16: 280; 
abs. in Pharm. Centralh., 42: 356. 
Ewart, A. J. (1) The action of chloroform on CO: assimilation. Ann. Bot., 
12: 415-417. 1808. 
Feltz, V. (1) Experiences demonstrant que le chloroforme n’a aucune action 
ni sur la septicite ni sur les vibrioniens des sangs putrefiés. Compt. 
Rend. d. Sci., 85: 350-351. 1877. 
Formanek, E. M. (1) Ueber die Einwirkung von Chloroform und Chlo- 
ralhydrate auf den Blutfarbstoff. Ztschr. physiol. Chem., 29: 416-422. 
1900. 
