ni REPORT OF THE DEPARTMENT OF BOTANY OF THE 
I. APPLE ROTS IN COLD Si ORR 
Since the commercial cold storage of apples has become such 
an important industry in New York State many questions relating 
to it have demanded solution. Many of these questions have been 
studied experimentally, and definite knowledge of great practical 
value regarding them determined. 
However, the question as to whether the several species of fungi 
that most commonly cause the decay of fruit will develop and pro- 
duce decay in fruit while held in commercial cold storage has never 
been studied. 
Since disputes over the condition of fruit held in commercial 
cold storage houses are frequent and sometimes find their way 
into court, it is highly desirable to have some reliable information 
as to the troubles that are directly traceable to the growth of cer- 
tain well known fungi. 
The methods of conducting the experiments upon this question 
were to obtain a good supply of pure cultures of the fungi, from 
these to make inoculations under sterile conditions into sound apples 
and to place the fruits at once in a commercial cold storage 
house. After remaining there a reasonable time they were re- 
moved into a warmer temperature. 
Experiments were carried on in the winter of 1905 in duplicate, 
but in different cold storage houses. Higher temperatures were 
also experimented with; one from 37° to 56° F., the average being 
47° F.; and another from 54° to 65.5° F., the average being 
60.6° F. The work with commercial cold storage at different tem- 
peratures was repeated in the winter of 1906 in one house. 
THE EXPERIMENTS OF 1905. 
A. good supply of vigorous pure cultures of bitter rot (Glom- 
erella rufomaculans (Berk.) Sp. & vonSchr.), black rot (Spherop- 
sis malorum Pk.), blue mold (Penicillium glaucum Lk.), brown 
rot (Sclerotina fructigena (Pers.) Schrt.), pink rot (Cephalothe- 
cium roseum Cda.) and Alternaria sp. were secured by taking ap- 
ples that were naturally infected with these fungi and with some 
of the mature spores making dilution or poured plate cultures with 
