New YorK AGRICULTURAL EXPERIMENT STATION. 125 
potato agar. rom these plates transfer cultures were made to 
sterilized sugar beet plugs and from these several sub-cultures 
were made. These last cultures were then tested to make sure of 
their pathogenicity. 
Sound mature apples of the following varieties were selected 
for the inoculations: Baldwin, Tompkins King, Northern Spy, 
Rhode Island Greening, Russet and Sutton. Each fruit was 
cleaned with a cloth and then dipped in a solution of corrosive sub- 
limate I : 1000; from this it was drained and rinsed in clean dis- 
tilled water, drained again and the excess moisture absorbed with 
a cloth that had been wet with corrosive sublimate and dried. 
Several fruits of each variety were inoculated with each spe- 
cies of fungus used. 
The inoculations were made on March 9, 1905. On each fruit 
the epidermis was punctured in three places with a sterile knife 
and with another sterile knife some of the fungus from a pure 
culture was inserted in the puncture. 
In connection with the inoculation experiments it was thought 
desirable to test the power of these species of fungi to grow in 
culture. This was done by preparing a quantity of petri dishes 
of sterile agar and transferring, under sterile precautions, some of 
the fungus from the pure cultures to several places upon the agar. 
The apples inoculated with a single species of fungus were put 
together and packed in a separate compartment in a bushel box. 
The plate cultures were wrapped in clean paper and packed in with 
the apples... Immediately after completing the work the boxes 
were taken by a special messenger to a commercial cold storage 
plant and at once placed in a room where the temperature was 
31° F. Records of the temperature in this room were taken six 
times each day at regular intervals. The record gave 360 readings 
during the time the experiment was in progress; 3 were 20°, I 
Wee, 07 Were 20°, 75 were 3032-5 143) were’ 31°,’ 27 “were 
Siac A Were 32°. 
The apples and cultures were all removed from the cold storage 
house on May g and examined that day. The results were as 
tollows: 
