New York AGRICULTURAL EXPERIMENT STATION. 133 
AN EXPERIMENT IN TEMPERATURE OF 35° TO 50°. 
_ An experiment similar to the previous one was made where the 
inoculated apples were placed in a temperature of from 35° ¢ 
50° F., the average being 47° F., determined by a maximum and 
mininum thermometer. 
The same species of fungi were used as in the previous work 
and the varieties of apples were King, Baldwin, Rhode Island 
Greening, Northern Spy, Russet and Sutton. 
The details of preparing the cultures, treating the apples and 
making the inoculations were the same as in the other experiments. 
After the apples had been in this temperature for five weeks they 
were examined and all- those inoculated with Alternaria sp., Glomer- 
ella rufomaculans (bitter rot) and Spheropsis malorum (black 
rot) had developed decay spots % to 1 inch in diameter at the point 
of inoculation. The decayed spots in the apple inoculated with 
Cephalothecium roseum (pink rot) were smaller. Three-fourths 
of every apple inoculated with Pemictllimm glaucum (soft rot) was 
decayed. The culture of Sclerotima fructigena (brown rot) which 
was used seemed to have lost its pathogenicity and the apples inocu- 
lated with this did not decay. 
Petri dish cultures on potato agar were also made at the time the 
apples were inoculated and kept in the same room with the apples. 
In all of these cultures good growth of the different species of fungi 
developed. 
At the end of the 5 weeks the apples were removed to a higher 
temperature and the decays developed rapidly. 
AN EXPERIMENT IN A TEMPERATURE OF 48° TO 60°. 
Another experiment was made, conducted in the same way as 
the previous ones, using a temperature of from 48° to 69° F., the 
average being 60.7° F., determined by a maximum and minimum 
thermometer. 
At the end of 3 weeks the apples inoculated with Alternaria sp. 
and Cephalothecium rosewm (pink rot) showed small decayed spots 
at the point of inoculation. 
Those inoculated with Spheropsis malo um (black rot), Sclero- 
tima fructigena (brown rot) and Penicillium saa (blue mold) 
were practically all decayed. 
Those inoculated with Glomerella rufomaculans (bitter rot) 
showed decayed spots I to 2 inches in diameter at the point of each 
inoculation. 
