136 REPORT OF THE DEPARTMENT OF BOTANY OF THE 
Repetitions of this experiment were made, using 4%, 1, 1% and 2 
pounds of sulphur. One and one-half pounds of sulphur burned in 
a tight room which was kept tightly closed for about 24 hours gave 
fairly satisfactory results, but 2 pounds of sulphur gave entirely 
satisfactory results. 
In this experiment there were used a large number of apples, 
which had been artificially inoculated and were producing an abund- 
ance of spores of the fungus. | 
After transfer cultures were made from all of these apples they 
were placed in a room which was made as tight as possible and the 
fumigation was done as follows: A large pan placed on the floor 
in the center of the room was filled with a quart or more of water, 
two bricks were set edgewise in this pan and on them rested a 
small tin pan which contained the 2 pounds of sulphur. This was 
easily ignited in the place where a teaspoonful of alcohol had been 
poured. The arrangement of the sulphur over water was done to 
eliminate any danger there might be from fire. 
The room was closed immediately after the sulphur was ignited. 
About 24 hours later it was opened. Cultures were again made 
from all the apples. After a reasonable time was allowed for their 
growth they were all examined. Those made before fumigation 
were growing vigorously while those made after the fumigation had 
not grown at all. They were all kept under observation for some 
time, but no growth developed in any cultures made after fumiga- 
tion. 
The experiments to destroy the spores of Penicillium glaucum 
(blue mold) in the air were made by fumigating with 1 and 1% 
pounds of sulphur to 720 cubic feet of space. The results with 1 
pound indicated that it was insufficient, but 11% pounds was used 
with good results. | 
The method was to secure a quantity of apples that were weil 
covered with the spores of Penicillium glaucum (blue mold), part of 
them being placed on shelves in the room where the fumigation was 
done, the others set on a plate, and by vigorous fanning the spores 
were blown all about the room. One-half hour later 12 petri dishes 
containing sterile potato agar were placed on the floor of the room 
and their covers removed for exactly 3 minutes. During this time 
the operator remained in the room so as not to cause any unneces- 
sary air currents by opening the door to go out. 
The fumigation was then done in the way previously described. 
The room was kept closed for about 24 hours. When opened the 
