New York AGRICULTURAL EXPERIMENT STATION. I51 
celled spores really belong to the Sporotrichum is proven by the 
fact that they have been observed repeatedly in cultures known to 
be pure. They have been observed in pure cultures of Sporotrichum 
poe and S. anthophilum on acidulated potato agar, plugs of sugar 
beet and various other media. The production of these unusual 
spores is to be regarded as strong evidence that the two fungi be- 
long to the same species. In none of the 142 species of Sporotri- 
chum described in Saccardo’s Sylloge Fungorum is there any men- 
tion of septate spores. 
In addition to the spores just mentioned two-septate and even 
three-septate spores are occasionally found in the carnation fungus 
and also in the June grass fungus in pure cultures as well as in 
nature. These are quite variable in size and shape. They are almost 
always longer than the once-septate spores and either straight or 
slightly curved. Sometimes they are plainly septate and quite Fu- 
sarium-like as shown in Plate XIII, fig. 4. In other cases the septa 
are indistinct and it may be uncertain whether they are spores or 
only spore-bearing branches of the hyphz which have become de- 
tached. 
It appears that it was the presence of these pluri-septate spores 
which caused Dr. Heald to refer his carnation bud-rot fungus to 
the genus Fusarium. It has already been stated that the four 
carnation buds sent by Dr. Heald as representative of his disease 
all showed an abundance of Sporotrichum with multitudes of the 
typical globose or broadly ovate spores; but one of the buds con- 
tained, also, a considerable number of the Fusarium-like spores. This 
particular bud showed a larger proportion of such spores than we 
have ever seen in any of the New York specimens; yet they were 
few as compared with the multitudes of typical Sporotrichum 
spores. So far as could be determined the mycelium was all of one 
kind and attempts to isolate the Fusarium by the dilution method 
failed notwithstanding the medium used was acidulated potato agar 
on which most Fusaria grow readily. The pure cultures obtained — 
produced about the same proportion of once-septate and pluri-sep- 
tate spores as the cultures obtained from New York carnations and 
June grass. 
Grown in pure cultures under parallel conditions the three fungi 
(S. anthophilum, S. pow and Heald’s figures) look and behave iden- 
tically alike.. On acidulated potato agar and on plugs of sugar beet 
at room temperature they grow readily producing a copious white 
mycelium and sporulating freely in from four to seven days. On 
