154 REpoRT OF THE DEPARTMENT OF BOTANY OF THE 
made no difference whether the cultures were grown in darkness 
or in sunlight. 
Hedgcock,® in his studies on chromogenic fungi which discolor 
wood, also found the chemical reaction of the medium to be an im- 
portant factor in color production. But it is noteworthy that with 
one species, Penicillium aureum Cda., the presence of an alkali 
favored the formation of red color instead of hindering it as was 
found by Smith and Swingle in the species studied by them. 
Returning, now, to our own observations —the carnation Sporo- 
trichum, the June grass Sporotrichum and Heald’s bud-rot fungus 
all three produce the same red color in cultures. On some media, 
_ especially plugs of sugar beet, this color may be depended upon to 
appear under almost any condition of light, temperature, acidity or 
alkalinity not incompatible with growth. On sugar beet, the color 
is somewhat more brilliant in darkness than in diffused light, and on 
acid media than on alkaline, but the differences are not great. At 
20° C, the time required for the appearance of the red color varies 
from three to seven days. It appears sooner on sugar beet than on 
potato, but ultimately it may become quite as brilliant on the latter 
as on the former. On the same medium and under apparently 
parallel conditions the color in different cultures varies considerably 
in shade. In occasional cultures no color ever appears. The three 
strains of Sporotrichum have been grown on various culture media 
and under various conditions for the purpose of finding cultural 
characters by means of which they might be separated, but the 
resulting variations in color, habit of growth, spore formation, etc., 
have been no greater than those commonly occurring between indi- 
vidual cultures of the same strain. 
The association of all three fungi with the same species of mite 
is at least suggestive of unity and finally, all three are parasitic on 
carnation buds. Heald has proven the parasitism of his fungus 
and the writer has done the same for Sporotrichum pow and S. 
anthophilum. . 
It having been proven that Sporotrichum pow and Sporotrichum 
anthophilum are two names for one and the same fungus, the 
former name should be given preference since it is the older. How- 
ever, it is by no means certain that the fungus has not been previ- 
ously described under yet a different name. 
* Hedgecock, George Grant. Studies upon some chromogenic fungi which 
discolor wood. Mo. Bot. Gard., Rpt., 17: 59-117. 1906. 
