156 REPORT OF THE DEPARTMENT OF BOTANY OF THE 
disease, which would account for the large percentage of failures. 
Later inoculations, made on Enchantress and Gov. Wolcott, were 
more successful. It is probable that in some of the experiments 
better results would have been obtained of the buds had not been 
cut so soon. It appears that from Io to 15 days are required for the 
fungus to destroy the buds in typical fashion. Buds in all stages of 
development, including fully expanded blooms, were successfully 
inoculated, but the best results were obtained with small, unopened 
buds. It is also plain that an abundance of moisture in the air is 
favorable to the disease. Inoculated buds on plants kept in a moist 
chamber after inoculation were soon conspicuously overgrown with 
the Sporotrichum and ruined; whereas, in the open greenhouse, 
under conditions otherwise parallel, the progress of the disease was 
slow and uncertain. This is as would be expected. 
Most of the inoculations were made with Sporotrichum isolated 
from carnations (S. anthophilum). Sporotrichum from June grass 
(S. po@) was used in three experiments, Nos. 11, 12 and 13, as 
follows: 
Experiment No. 11.—On Nov. 16, 1907, two buds (one showing 
color and the other of large size but unopened) of Gov. Wolcott 
were inoculated with a pure culture of Sporotrichum isolated from 
June grass July 5, 1907. The inoculations were made in the usual 
manner by forcing spore-laden water through a hole in the side of 
the calyx. (The Sporotrichum spores were already germinating, 
having been placed in the water 20 hours previously.) After in- 
oculation the plants were kept in a moist chamber where the tem-. 
perature was about 13° C. by night and 21° C. by day. There were 
no checks. 
At the end of two weeks the buds were cut and examined. The 
more adanced one was now fully expanded. It was evident that 
the fungus had taken effect. The pistil and bases of the petals 
had been destroyed, but owing to the splitting of the calyx which 
permitted the petals to hang apart, the upper portions of the petals 
were unaffected. The other inoculated bud was half open. Viewed 
from the top, about half the petal tips appeared normal while the 
remainder were brown with decay. On the interior of the bud 
the petals were badly decayed and glued together, the tissues being 
thoroughly permeated with Sporotrichum hyphe fruiting moder- 
ately but not forming a conspicuous mold. In this bud the petal 
bases were unaffected. 
Expermment No, 12. —On Nov. 18, 1907, ten buds, (5 show- 
