New YorK AGRICULTURAL EXPERIMENT STATION. 157 
ing color and 5 large but unopened) of the variety Boston Market 
were inoculated with Sporotrichum from June grass exactly as in 
Experiment No. 11 except that the spores were not so far advanced 
in germination, having been placed in water but six hours before 
the inoculations were made; and the plants instead of being placed 
in a moist chamber were left in the open greenhouse. At the same 
time, in the same manner and under exactly parallel conditions, ten 
other buds were inoculated with Sporotrichum from carnations. 
There were also five check buds (showing color) which were in- 
jected with sterile water. 
At the end of 15 days all of the buds were cut and examined. 
Of the 5 buds showing color at the time of inoculation with June 
grass Sporotrichum, four had fully expanded and partly withered 
without evidence of injury from the fungus, but the fifth was only 
half open, badly decayed within and presenting a typical case of 
Sporotrichum bud-rot. Of the 5 unopened buds inoculated with 
June grass Sporotrichum, one had been destroyed by some animal, 
three were fully expanded and showing only slight injury while the 
fifth was completely ruined by Sporotrichum. 
The inoculations with carnation Sporotrichum were more success- 
ful. The 5 buds showing color at time of inoculation gave negative 
results except that in three cases the pistil had been destroyed; but 
the 5 buds which were yet unopened at time of inoculation were now 
about half open, much decayed in the center, somewhat moldy and 
all good typical examples of Sporotrichum bud-rot. 
The five check buds had expanded normally without any discol- 
oration or other evidence of injury. 
The details of Experiment No. 13 are given on page 158. 
No mites have been found in any of the inoculated buds. It is 
possible that the mites in addition to serving as distributors of the 
fungus may assist the fungus in making its attack on the tissues. 
There being no evidence that the fungus attacks anything but 
the buds no attempt has been made to inoculate it into any other 
part of the carnation plant. 
Notwithstanding numerous failures, the 36 successful inoculations 
described above seem to prove conclusively that the Sporotrichum 
found in carnation buds affected with bud-rot is a parasite and the 
cause of the disease. 
