158 REPORT OF THE DEPARTMENT OF BOTANY OF THE 
EXPLCANATION@ Ol PipAser 
A and B were two buds of the same age on the same carna- 
tion plant, variety Enchantress. When the buds were of the size 
of C and just beginning to open, B was inoculated with a pure 
culture of Sporotrichum poe isolated from a diseased carnation 
bud two months previous. Inoculation was affected by forcing into 
the bud (through a hole made in the calyx) water containing 
spores of the fungus. A, used for a check, was similarly injected 
with sterile water. A bell jar was then placed over the plant. 
Twenty days later the two buds were cut and photographed, natu- 
ral size. A was then a perfect bloom, while B was completely 
ruined. In the latter, all parts except the calyx were decayed and 
overgrown with a fine, white, cottony growth of the Sporotrichum 
which was sporulating freely. 
