180 REPORT OF THE DEPARTMENT OF BOTANY OF THE 
and size of the plants was so striking that the boundary of the 
inoculated strip could be followed by the eye the whole length of 
the field. (See diagram on page 181.) On the inoculated strip 
there was a rank growth of dark green color. The plants were 17 
to 24 inches high, most of them were robust and much branched, 
and they covered the ground completely. On the adjacent uninocu- 
lated portion of the field there was a lhght growth of golden yellow 
color. The plants were 12 to 17 inches high, slender, mostly un- 
branched and the ground among them was plainly visible. The 
yellow plants did not show any dead or spotted leaves — they were 
simply golden yellow throughout. However, this condition did not 
prevail over all of the uninoculated portion. On a strip about 50 
ft. in width and extending from the south end about one-third 
of the distance across the field near the east side, the alfalfa was 
nearly as large and as good color as on the inoculated strip. A 
satisfactory explanation of this could not be found. This part of 
the field was a little lower than the rest. Scattered over the re- 
mainder of the uninoculated portion were occasional small clumps 
of thrifty, dark green plants which contrasted strongly with the 
yellow plants about them. Such clumps were especially numerous 
ina line extending from the low area above-mentioned to the north 
end of the field. This is shown in the diagram. 
Upon making an examination of the roots it was learned that 
the dark green plants, wherever found, were well supplied with 
nodules which often occurred in conspicuous whitish clusters one- 
half inch or more in diameter; .while the yellow plants, even when 
standing close beside green ones, appeared to be wholly devoid of 
nodules. 
Mr. Colyer states that the first cutting was made July 1, when 
the alfalfa was commencing to bloom. On the inoculated strip 
(A) and the low area (C) there was a heavy yield of hay, but 
no weights were taken. The second cutting was made on August 
g and 13. On the latter date we made a second examination of 
the experiment. This time there was a fairly good crop of hay 
on the inoculated strip and the low area, while over the remainder 
of the field the plants were short and there were many bare spots. | 
The contrast in color had now entirely disappeared. The whole 
neld was of the normal green color. However, it was evident that 
many of the yellow plants had died. ‘The nodules, also, had mostly 
disappeared. A few live, plump nodules were found and some of 
the clusters were still in evidence, but most of them were shriveled 
and dead. A severe drought prevailed at this time and the ground 
