238 REPORT OF THE DEPARTMENT OF BOTANY OF THE 
usually bi-guttulate. Many of the spores are apparently non-sep- 
tate. The majority of them are slightly constricted at the middle 
and many are certainly once-septate, but it is often difficult to make 
out the septum. Sometimes one of the cells is a little broader than 
the other. 
The Diplodina grows readily on ordinary culture media such as 
sterilized plugs of sugar-beet and acidulated potato agar. On sugar 
beet the growth soon becomes black with enormous numbers of 
pycnidia which are filled with multitudes of spores. 
This fungus closely resembles the Ascochyta occurring on alfalfa 
leaves but it is certainly a different species. (See page 225.) 
MISCELLANEOUS. 
AN ALFALFA PLANT WITH UNFOLIATE LEAVES. 
In alfalfa, the first leaf after the seed-leaves consists of a single 
leaflet while the succeeding ones are pinnately trifoliate.** (See 
Plate XXI.) Recently, the writers found an alfalfa plant having 
unifoliate leaves throughout. There being, apparently, no pub- 
lished record of such a freak alfalfa plant it is thought worth while 
to give an account of it here. 
The plant was discovered among some alfalfa seedlings grown 
in the Station greenhouse during the spring of 1907. It grew in 
a flower pot in the greenhouse until the spring of 1908 when it 
was transplanted into the open garden together with four other 
plants propagated from it by cuttings. At the present writing 
(October, 1908) all five plants are in fairly normal condition. In 
general appearance they are similar to normal alfalfa plants. None 
of them have never shown anything but the unifoliate leaves which 
are of the same size, shape and appearance as the terminal leaflet 
in ordinary trifoliate leaves. The plants have flowered only spar- 
ingly and produced no seed. They have been affected with leaf 
spot (Pseudopeziza medicaginis), and Diplodina medicaginis ap- 
peared on some of the dead stems in October, 1907. 
MULTIPLICATION OF LEAFLETS. 
We have occasionally seen alfalfa leaves with four and five leaf- 
lets. 
The same is true of other species of Medicago, Trifolium and some 
other Leguminose. (See Lubbock, Sir John. A contribution to our knowl- 
edge of seedlings. 1:388). 
