78 Report oF DEPARTMENT or BACTERIOLOGY OF THE 
TABLE IV.— Errect or INcLosep AIR IN. THE TUBES WHILE IN THE SALT 
SOLUTION. 


Air left in tubes. Air out of tubes. 
eee —————————————————————————— — eee 
Both cows Cows milked Both cows Cows milked 








1909. milked at once. separately. milked at once. separately. 
Prin- | Millie | Nora | Carey | Prin- | Millie | Nora | Carey 
cess A. | F. B. B IBY of S. cess A. ioepihs D: of 8. 
Germs per cc. in milk. 
JUNG 2) Fa seem eee ee kee ce and mee See 1,500 1,780 930 "| \ea ee ae ee eee 
ae pes 10,720 42600 0s Es thoes Ree eo Peel en ener eee 2,700 1,760 
Fe Mth. L400 ST FLG. S/O eH Se ee ee A Oc gee, See aCe oe teams Ce ea enmcen ne hat) 1,790 
Kai (Od 1G SA 90.8.3 1BB0 TT Peas ait) hee Heee es Mee eC eee ee 3,660 1,750 
Pe 2s a Pedeee eee Meee 4: bench 1,600 2,000 27540 100 oa oy Re eee 
ae PA oes 1,730 2 ABO! PT CEPA MAY he hose eal nee cone | ae eee 1,620 1,450 
AV 5,070 6, 700 1,600 1,750 2,070 660 2,930 | 1,690 
Gen. av 4740 1990 
The data in the above table came as a surprise, since, if we 
eliminate the two high counts on May 28 and 29, there is prac- 
tically no difference in the results obtained by the two lines of 
treatment. 
On one hand is the observed marked improvement which 
immediately followed the careful removal of all air from the 
tubes at the beginning of our work and on the other is the 
failure of larger numbers of bacteria to appear in the milk 
when the air was purposely allowed to remain in the tubes. 
In the face of such conflicting evidence positive conclusions 
can not be drawn but it does seem a logical conclusion that 
since the action of the brine was so important in reducing the 
germ content its free access to the entire surface of the tube 
should be followed by beneficial results. 
FILTERS 
While the use of brine for holding the teat cups and rubber 
parts between milkings very markedly improved the quality 
of the milk as shown by the reduced germ content, even under 
