104 Report or DEPARTMENT OF BACTERIOLOGY OF THE 
nip Rot D, was isolated at the Vermont Agricultural Experi- 
ment Station laboratory from decaying turnips growing on 
the same land from which were obtained the cabbage BOn rot 
organisms XX V—XXXI two years previously. 
At the New York Agricultural Experiment Station labora- 
tory culture 0.2 e was isolated from a decaying cauliflower 
from Long Island in November, 1901. Culture 0.2 f was iso- 
lated in March, 1902, from a diseased cauliflower plant in 
the green house. Eleven days previous this cauliflower plant 
had been carefully inoculated with a pure culture of 0.2 e in 
order to test the pathogenicity of this culture and the re- 
isolation was made to determine the fact that the disease 
which had been produced was actually caused by 0.2 e. The 
inoculation had been made as aseptically as possible and the 
point of inoculation covered with melted grafting wax. The 
material for the reisolation was taken from the advancing 
edge of the diseased area and the plates indicated the presence 
of a pure culture in the plant tissue. 
In 1902 the cultures designated, as Miller and Riverhead 
were isolated from decaying stalks of cabbage which had 
been planted commercially for the purpose of raising cabbage 
seed. The stalks were collected as typical examples of the 
early stages of the destructive rot which causes a heavy loss 
in the cabbage seed industry on Long Island practically every 
year and there were two stalks from each of two fields located 
about 10 miles apart in the eastern portion of the island. 
Cultures labeled Miller Stalk 2 No. 1 and Miller Stalk 2 
No. 2 were derived from the same diseased stalk while Miller 
Stalk 3 No. 1, Miller Stalk 3 No. 2 and Miller Stalk 3 No. 3 
were likewise isolated at the same time from another sim1- 
larly diseased cabbage stalk from the same field. Riverhead 
Stalk 2 No. 1 and Riverhead Stalk 3 No. 1 came from two 
cabbage stalks out of a second field. 
Cultures 0.2 RBe and 0.2 RBi were isolated in November, 
1901, from a rutabaga. This was obtained from a private 
garden near Phelps, N. Y., and the upper portion of the ruta- 
-baga was soft and vile smelling. The cultures were prepared 
from blackened fibro-vascular bundles passing through the 
