128 Report oF DEPARTMENT OF BACTERIOLOGY OF THE 
tion and this group with a group number of B. 221.2113022 
is represented by Vermont LIV and 0.2e. The faintness of 
the boundaries between these groups is shown by the case 
of 0.2e where in two determinations gas was actually formed 
in a single fermentation tube in each case. This result has 
not been accepted as conclusive because in neither case were 
the results from the accompanying tubes accordant. 
The next group bears the group number B. 221.2123022 and 
is represented by Potter’s Bacillus and Riverhead Stalk 
3 No. 1. The former is a good illustration of the group while 
the latter forms a connecting link with the following group, 
since while it formed visible gas from lactose in the earlier 
tests it failed to do so during the latter studies and but for 
the earlier determinations would be included in the following 
group. 
The series of groups is closed with a group of four cultures 
in which there was no visible gas formed from any of the 
sugars at any of the tests. This has a group number of 
B. 221.2223022 and Bacillus aroideae Townsend, is the only 
named species. Spieckermann’s Bacillus was isolated and 
described at an earlier date, but was not given a specific name. 
Summarizing this arrangement on the basis of the results 
from the fermentation tube test with dextrose, lactose and 
saccharose we have the following: 
B. 221.11138022 Bacillus carotovorus Jones and 32 other 
cultures. 
221.1123022 ° Vermont XLVIII. 
221.1218022 Vermont C. 
221.21138022 Vermont LVI and 1 other culture. 
221.2128022 Potter’s Bacillus and 1 other culture. 
221.2223022 Bacillus aroideae and 3 other cultures. 
PRA 
It will be observed that the two possible groups of .221 
and .122 are not represented. It is really surprising that in 
a collection of 48 cultures there should have been examples 
of six different groups and there is no apparent reason why 
a study of a larger number of cultures should not bring out 
these missing ones. 
