Hm CO 
“1S Ot 
ea) 
New YorK AGRICULTURAL EXPERIMENT STATION. eal 
Bacillus carotovorus Jones. 
For group number and brief characterization see page 110. 
I. MORPHOLOGY. 
1. Vegetative cells. Medium used, agar slope at 20-25° C. 
1-2 days old; Form, short rods, long rods, short chains, 
long chains; Limits of size, .7 to 1x 1.5 to 5; Size of 
majority, .8 by 2; Ends, rounded. 
. Endospores, none. 
. Flagella, 2 to 10; Attachment, peritrichiate; How stained, 
Pitfield, Lowits, Fischer. 
. Capsules, none. 
Pseudozoogloea, present, slight. 
. Involution forms, not observed. 
. Staining reactions. Stains well in watery fuchsin, gentian 
violet, carbol fuchsin, Loeffler’s alkaline methylene blue ; 
Gram, negative. 
II. CULTURAL FEATURES. 
. Agar slope. Growth, abundant; Form of growth, filiform 
to spreading; Elevation of growth, effuse to raised; 
Luster, glistening; Typography, smooth to contoured; 
Optical characters, opaque to opalescent. 
. Potato. Growth, moderate to abundant; Form of growth, 
filiform to spreading ; Elevation, effuse to raised; Lustre, 
glistening; Typography, smooth to contoured ; Chromo- 
genesis, white on all media; Odor, decided ; Consistency, 
butyrous; Medium, grayed, but not discolored in the 
customary meaning of that term. 
. Agar stab. Growth, best at top, abundant, wide-spread- 
ing; Line of puncture, filiform. 
. Gelatin stab. Growth, best at top; Line of puncture, fili- 
form; Liquefaction, crateriform to infundibuliform ; Be- 
gins on 2d day at 20° C.; Complete in 6 days with some 
cultures and not in months with others. 
