ae REPORT OF DEPARTMENT OF BACTERIOLOGY OF THE 
6. Nutrient broth. Surface growth, pellicle thin and some- 
times absent; Clouding, moderate to strong, persistent ; 
Odor, decided; Sediment, compact to flocculent, usually 
abundant. 
7. Milk. Coagulation, usually on 3d day at 20° C.; Coagu- 
lum, very slowly and slightly peptonized, and not com- 
plete in months, digestion not clearly evident to the 
eye; Medium, slightly browned. 
8. Litmus milk. Acid, litmus reduced. 
9. Gelatin colonies. _ Growth, rapid; Form, punctiform to 
round; Depression, crateriform; Edge, entire; Lique- 
faction, saucer. 
10. Agar colonies. Growth, rapid at 20-25° C.; Form, round, 
occasionally irregular, deep colonies fusiform; Surface, 
smooth; Elevation, raised to conver; Edge, entire to 
undulate; internal .structure, amorphous to coarsely 
granular or even grumose. 
13. Cohn’s solution, no growth. 
14. Uschinsky’s solution, abundant. 
18. Best medium for long continued growth is peptone 
bouillon. 
19. Quick test for differential purposes. Slices of uncooked 
carrots, turnips and cabbages. 
III. PHYSICAL AND BIOCHEMICAL FEATURES. 
1. Fermentation tubes. Gas** produced in small amounts 
from dextrose, lactose and saccharose, but not from 
glycerine; Growth in the closed arm with dextrose, 
lactose and saccharose, but not with glycerine; Acid pro- 
duced from dextrose, lactose, saccharose and glycerine. 
3. Nitrates in nitrate broth reduced to nitrites. 
4, Indol production feeble. 
7. Optimum reaction for growth in bouillon in terms of 
Fuller’s scale, 0. 
* See page 117. 
