New York AGRICULTURAL EXPERIMENT STATION. 159 
At alcoholic strength of 80%, secured 80% of total pre- 
cipitate. 
At alcoholic strength of 90%, secured 5% of total pre- 
cipitate. 
Trial of the last three precipitates (i. e., 40%, 80%, 90%) 
on carrot sections showed all to have cytolytic activity, but 
that from the 90% had distinctly less than did the others. 
Between those of 40% and 80% there was no noteworthy 
difference. In a second trial the culture broth was divided 
into three lots of 150 c. c. each, and each lot treated sepa- 
rately as follows: 
Lot 1, made 40% alcoholic, gave 0.005 g. precipitate, or 1% 
of total. 
Lot 2, made 60% alcoholic, gave 0.065 g. precipitate, or 14% 
of total. | 
Lot 8, made 80% alcoholic, gave 0.390 g. precipitate, or 85% 
of total. 
Trials of these precipitates on carrot and radish sections 
Showed those from lots 2 and 3 to be of excellent activity and 
about alike, whereas that from 1 required twice as long to 
soften the sections. 
Since in both of these trials the 80% alcohol secnred prac- 
tically all of the enzym, and this in a state of the highest 
activity, that strength alone was used in all subsequent alco- 
holic precipitation work. , 
3. Reprecipitation.—The precipitate obtained by the addi- 
tion of alcohol to the broth is, of course, composed only in 
part of the enzym, the larger portion being presumably other 
proteid matter. In the hope of securing a purer state of the 
enzym, a re-solution and second precipitation with alcohol was 
made as follows: ; 
Two grams of the dry powdered precipitate obtained from 
beef broth cultures was added to 400 c.c. of distilled water, 
the solution placed on ice and frequently shaken. At the end 
of four hours one-half was filtered off and, since filtering 
through paper did not remove the undissolved precipitate, it 
was clarified by passing through a porcelain filter, then re- 
precipitated by rendering 80% alcoholic. The balance stood 
