162 Report oF DEPARTMENT OF BACTERIOLOGY OF THE 
To test this matter a portion of the precipitate from a six 
day beef broth culture was added to a volume of water equal- 
ling that of the original broth (0.015 g. of precipitate in 5 
c. ¢. of water, equalling 0.3% solution). The activity of this 
solution was then compared with that of a like broth culture 
sterilized by the addition of chloroform, and of third steril- 
ized by passage through a Pasteur-Chamberland filter. The 
results were that the first and second showed practically the 
same degree of enzymic activity, whereas the third showed 
less than half as much. These comparative trials were re- 
peated on a later occasion. Here we used (a) the juice ex- 
pressed from a turnip decayed by B. carotovorus, inoculated 
three days previously; (b) an aqueous solution of the alco- 
holie precipitate obtained from a similarly decayed turnip, so 
made up that the volume of water equalled that in the original 
juice from which the precipitate came. No chloroform was 
added to (a), the living culture being used. Trials of these 
on turnip and carrot sections showed the enzymic activity 
of (b) to be fully as great as that of (a). The results of 
Spieckermann (1902: 146), which reached us after the earlier 
trials were made, are in 2ccord with these. It is surprising 
that no loss occurs through precipitation and re-solution of 
a compound so se: sitive as this enzym shows itself to be in 
many ways. é 
In these trials the original broths and the aqueous solutions 
of a strength equalling them have proved on the average less 
than one-third as active as the 5% solutions which we,have 
used in most of our trials with the precipitate; that is to say. 
these 5% -solutions have rotted the vegetable sections in less 
than one-third the time required to do this where the sections 
are immersed in the living cultures. 
RELATION OF CULTURAL CONDITIONS TO ENZYM 
PRODUCTION. 
THE MEDIUM. 
The vigor of growth of the organism varies widely, of course, 
with the composition of the medium and with other cultural 
