New York AGRICULTURAL EXPERIMENT STATrIoNn. 197 

Oo 
Fic. 8. Camera drawings of margin of a very thin section of carrot root 
tissue; a, normal. a’, same 13 minutes after immersion in 5 per cent. 
solution of enzymic precipitate of B. carotovorus. (See also Fig. 4 where 
same solution was used.) Since the projecting wali fragment at 0' was 
most fully exposed to the cytohydrolytic action it was carefully measured 
to determine whether it would undergo any shortening. The enzym solu- 
tion was renewed, with the addition of chloroform to inhibit bacteria, and 
the slide kept under observation for three weeks, but no further solution 
occurred. 
C oe 
a 
in 
a b oe 
Fig. 9. Similar camera drawings of a bit of the cell wall projecting 
from the margin of a thin section of carrot-root tissue. The normal wall 
is shown in a; b, the same after 20 minutes action of 2.5 per cent. aqueous 
solution of taka diastase; c, after 22 hours action, showing the gradual 
solution of all parts, the middle lamella being more persistent. 
seta : 
d 
, A 
wT @ a Eb 
Fic. 10. Similar drawings from another preparation; normal carrot 
walls shown in a; b, the same after 2 hours action of 2 per cent. solution 
of taka diastase. The continuous lines show the more persistent middle 
lamellae. The innner lamellae were entirely dissolved in places. 

