New YorK AGRICULTURAL EXPERIMENT STATION. 203 
Newcombe (1899) made a comparative study of cytolytic 
enzyms in the course of which he repeated and verified the 
observations of Brown and Morris upon the barley enzym and 
secured similar soluble ferments from some other germinating 
seeds. He found the enzymic extract from the cotyledons of 
Lupinus albus to be strongly cytolytic and but feebly amylo- 
lytic. Extracts from both the cotyledons and the endosperm 
of Phoenia dactylifera showed cytolytic and amylolytic activ- 
ity, and here again the cytolytic action was relatively greater. 
There was, however, a noteworthy difference between the two 
date-seed extracts in’that the one from the cotyledons had 
much greater amylolytic strength and relatively weaker cyto- 
lytic activity. This showed that the action on starch and the 
action on cell wall were distinct and independent processes. 
This evidence was further strengthened by comparisons of 
these with barley malt extract and with taka-diastase, both of 
which were found relatively stronger in amylolytic action. 
This seems conclusive as against Reinitzer’s contention that 
the cytolytic action was due to diastase. nae 
Further comparisons showed also a lack of correlation be- 
tween the rate of solution of the middle lamella and inner 
lamellae, respectively. Thus the five solutions were made to 
such a strength that all showed a like rate of action on starch. 
Sections of barley endosperm were then immersed in them and 
the periods necessary for complete solutions of inner and of 
middle lamellae, respectively, in each found to be as follows: 

Solution Solution 
; of of 
Source of extract. inner lamella. middle lamella: 
Lupinus.cotyledon.....4....- required 9 hours... . .required 21 hours. 
Date endosperm............. Y OLS ee f. ¥ LS 
Da CeCORVECCLOM ek. aks a bad dein be Papal aa : VS 
KA -GlAStase ote. nel. Seo e's a 94-116 ive: e- OVENS Low 
OES TE eS, ee ae 94-116 “ Eh ae 4 UEDA te 


These figures also serve to indicate the difference in the 
relative amylolytic and cytolytic activities discussed above. 
Green in the second edition of his work on Fermentation 
(1901 :105) cited these observations of Newcombe’s as confirm- 
