258 REPORT OF THE BOTANICAL DEPARTMENT OF THE 
ous in their early stages, and biguttulate to sparsely septate, 
later. 
Squash agar, potato agar, squash plugs and stems were used 
as culture media. ‘‘ When growing on plugs or stems of squash, 
the mycelium is whitish and floccose; but with age changes to 
a dark color. Experience has shown that, on the rind, the pyc- 
nidia are formed most abundantly.’’® 
“Seedlings of several varieties of squash, pumpkin, cates 
loupe, watermelon and cucumber were inoculated with fresh 
spores during the winter and spring months. This was done 
not only with the fungus found growing on the individual host, 
but also cross inoculations were made on the other hosts of 
the cucurbits. The results were most satisfactory and con- 
clusive 7. e. that all these several fungi were really one and 
would grow on any of the cucurbits used in the experimental 
work.”?? “The Ascochyta form of the fungus, grown from the 
ascosporic stage, was found to cause the disease on cucurbits 
like the other Ascochyta grown from Ascochyta spores.’’?°® 
“ Also a species of Ascochyta, resembling the one studied, 
grow[n] from an ascosporic or perfect stage, a species of 
Sphaerella, gave equally positive results in inoculation work.’’?+ 
Mr. Smith expresses the view that since an Ascochyta differs 
from a Phyllosticta or Phoma only in its having septate spores, 
it may easily be mistaken for one of the continuous spored 
genera. He thinks it probable that Phyllosticta orbicularis E. 
& E.?? “may be the same fungus.” 
A Mycospherella associated with pycnidia.—A Mycosphe- 
rella was sometimes found associated with the Ascochyta on 
squash stems. ‘ Attempts were made to germinate the ascos- 
pores . . . but only on one occasion were they seen to 
germinate. . . . Agar plates were poured, and these were 
inoculated at several points with a single perithecicum of the 
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2 The N. Am. Phyllostictas (1900). 
