266 REPORT OF THE BOTANICAL DEPARTMENT OF THE 
while those from the cortical region developed fungous my- 
celium in every case. 
Preliminary inoculations in the greenhouse.—There being 
still some unaffected plants in the greenhouse, a few of them 
were used for preliminary inoculations. Two of the healthy 
vines were inoculated at two points each, by cutting a bit of 
the cortical tissue loose and inserting bits of mycelium from 
the potato-agar tubes, taking the usual precautions regarding 
sterile instruments. After sterilizing the scalpel carefully, 
two additional wounds were made. All were covered with 
grafting wax. Three days later both the inoculated vines were 
slightly wilted, and a week after the date of inoculation the in- 
fested vines had the characteristic appearance of the disease 
both above and below all four of the inoculated points; the usual 
oily-green, water-logged appearance of the affected regions 
farthest removed from the points of infection, and drops of 
gum on the nearer, darker colored parts. Next the wax, all the 
vines had an abundance of the yellowish brown to dark brown 
fruiting bodies. The checks, on a vine next the inoculated ones, 
had no trace of disease, and upon removing the wax from one, 
it was seen that a normal callus had formed over the wound. 
The parasite may penetrate uninjured melon vines.—On 
August 2 a few inoculations were made in the greenhouse to 
test the penetration powers of the parasite. A healthy vine was 
selected and bits of mycelium from the formerly used set of 
pure cultures were placed on the uninjured surface of the main 
axis; three in crotches and one on an internode. August 8 the 
fungus had evidently penetrated at two of the inoculated points, 
for the usual water-logged appearance on both sides of the 
covering of grafting wax was present. About two weeks later: 
(August 22) the disease had progressed some distance above 
and below the place of infection at both of the above noted 
cases, but the one at the node seemed to have penetrated deeper 
than that at the internode. The nodal infection looked darker 
and had the usual quantity of resin-like gum on its surface, 
while the one at the internode left the vine looking a little 
light-grey, with scarcely a trace of gum. But both had fruit- 
