270 REPORT OF THE BOTANICAL DEPARTMENT OF THE 
LABORATORY CULTURES OF THE FUNGUS (IN 1907). 
Cell cultures of pycnidiospores.—A number of culture cells 
were prepared with small drops of sterile water and a few (per- 
haps from three to twenty) pycnidiospores were introduced, 
which were secured by putting some crushed pycnidia into a 
test tube about one-third full of sterile water. Twenty-four 
hours after planting nearly all were germinated. Some camera 
drawings on Plate X will help one get an idea of some of the 
stages as they occurred at this time. At the close of the 
second day there was some additional growth and branching, 
but the third day gave scarcely any growth although some 
differentiation had taken place. The short and_ sparsely 
branched mycelia had become septate and’ in many cases only 
a few of the terminal cells were filled with protoplasm. Most 
of the proximal cells seemed practically empty. Occasionally 
the two terminal cells on short lateral branches looked spore- 
like by being almost constricted off from the other cells of the 
mycelium. It has not been shown whether these bodies are 
secondary conidia or not; perhaps they are not. These cul- 
tures simply show that the pycnidiospores germinate in water 
and may be able to infect the host from twenty-four to thirty 
hours after a suitable environment has been attained. Trans- 
fers were made to potato-agar tubes to use for inoculations. 
Cell cultures of ascospores.—Another set of fifteen culture 
cells were prepared like the above and the drops were each 
supplied with a crushed perithecium. Five infected drops were 
allowed to evaporate by holding the cover glass at some dis- 
tance above a flame. <A bit of agar was then put on the dried 
asci. All cultures were examined and those discarded which 
had no asci with spores. Both fusoid and constricted spores 
were often in the same drop, but in different asci. Two days 
after preparing the cultures many of the spores had germinated. 
There was practically no difference between the germination of 
these ascospores and the pyenidiospores. None of the fusoid 
spores were seen to germinate. The drops from three of the best 
cells were transferred to five potato-agar tubes. On the follow- 
ing day many of the germ tubes in the remaining culture cells 
