272 . Report oF THE BOTANICAL DEPARTMENT OF THE - 
were, with few exceptions, without spores. Those having spores 
had the characteristic appearance, and were of the same size 
as those used for the cultures. The three checks for each group 
were normal and uninjured throughout. 
Inoculation of pumpkin vines with both mycelia.—To get 
some idea regarding the possible parasitism of this fungus for 
other species of the gourd family, pumpkin (Cucurbita Pepo) 
cucumber (Cucumis sativus), and watermelon (Citrullus vul- 
garis) plants were inoculated with both types of mycelia. 
On August 25 a vigorous pumpkin plant was inoculated 
in the usual way on various parts of its vine. Three inocula- 
tions with pycnidiosporic and four with ascosporic mycelium 
were made. September 5 two of the pycnidiosporic and three 
of the ascosporic infections had rather narrow bands of slightly 
rater-logged tissue on both sides of the wax covering, but no 
traces of gum flow could: be seen. September 14 they were 
again examined. The wax was removed from those having 
shown no trace of the action of the parasite. 
The wounds made during inoculation had healed normally. 
The narrow bands of water-logged tissue on those mentioned 
above had become no wider but had become dry. <A few of the 
covers were removed and a dead girdle of cortical tissue was 
found extending about the width of the wax. The more or less 
hollow pith region of one of these had the inner surface of its tis- 
sues of a. brownish color. Some transfers were made of the pith 
tissue from two other specimens, which had had waterlogged 
areas about the wax, and a fungous growth resulted which 
seemed indistinguishable from the one employed in the inocula- 
tions. On September 18 there were scattered perithecia on the 
three remaining infections around which some dead tissue could 
be seen. The hollow pith region (around the edges of which 
was some brown tissue containing hyphae of the fungus). as 
well as the perithecia, may be seen on Plate XII. Upon con- 
sulting the labels attached to the above inoculations two were 
found to have been infected with ascosporic and one with 
pycnidiosporic mycelium, on August 25. The perithecia were 
of the usual type, size and color. There were no pycnidia 
