216 REportT OF THE BOTANICAL DEPARTMENT OF THE 
pycnidiospores by placing the spores in a wound and covering 
with grafting wax. The pycnidia for this were carefully picked 
with a sterile needle and crushed in small drops of sterile water 
on sterile slides. The drop containing the spores was: then 
carefully drawn off into the wound. One check was prepared. 
September 18, three of the plants were very much wilted and 
the fourth had only a small region next the wax which was 
water-logged. The three dying plants had numerous pycnidia, 
but no perithecia, on the diseased parts. On September 26 the 
fourth inoculated plant had wilted and was dying. Pycnidia 
were also present. October 7 perithecia could be found on two 
of the four. The check was still in good condition. On Septem- 
ber 25. the two remaining potted plants were inoculated with 
ascosporic mycelium which had been obtained from Van Tieg- 
hem cell cultures, September 18. October 7 both vines were 
wilting and dying, and there were pycinidia present near each 
point of inoculation. 
The relation of the two types of fructification.—The pure 
cultures from the ascospores and the pycnidiospores, used in the 
foregoing inoculations, give strong evidence that the Diplodina 
pycnidia are the fruiting bodies of the Mycospherella, coming 
later. There seems no question but that these two types of 
fruiting bodies are simply stages in. the life cycle of the same 
fungus. With but one exception, inoculation with ascoporie 
mycelium produced the Diplodina pycnidia before any perithecia 
were produced. The exception has but little weight here be- 
cause on that host (pumpkin) the pyenidiosporic inoculation 
produced only perithecia also. 
SPORES, IN CULTURE, NOT TYPICAL. 
On November 12, transfers of both types of mycelia were 
made to tubes of alfalfa stems. The mycelial growth of both 
was similar and vigorous, turning darker with age. November 
26, numerous typical Diplodina pycnidia were present in all 
the tubes. No difference could be detected between those from 
the ascosporic, and pycnidiosporic mycelia; they were some- 
what undersized from both. The spores were also small, 
