New YorK AGRICULTURAL EXPERIMENT STATION. 279 
MYCELIAL INOCULATIONS OF SOME OTHER SPECIES OF CUCURBITS. 
Several other cucurbits were specially grown (1908) to be 
used in pathogeneity tests. Young potato-agar cultures were 
used as sources of the fungous mycelium. The stems of the 
plants were wounded by tangential cuts and the mycelium was 
placed under the sliver. About half of the inoculations were 
covered with grafting wax and the others were left without: 
protection. (Both methods were effective to about the same 
degree.) In most cases but one set of inoculations was made- 
before bacterial contamination of the stock cultures became 
evident, as shown by the inability of the fungous mycelium to 
parasitize Cucumis Melo. The following is a brief summary of 
the results : 
1. Cucumis sativus (Cucumber). Twelve inoculations were 
made as stated above. No visible effect was produced on the 
host and no fungous fruits were developed. 
. Cucumis Anguria (West Indian Gherkin). Ten inocula- 
Hai were made. The wounds healed normally and no fungous 
fruits resulted. 
3. Cucurbita maxima (Boston Marrow Squash). Eight in- 
oculations were made on the vines and six on the fruits. The 
wounds healed normally. However, some perithecia with spores 
and a few pycnidia appeared on the dead slivers of the vines, 
but the squash-fruits seemed neither affected by such inocula- 
tions, nor developed any fungous fructifications. 
4. Cucurbita Pepo (Pumpkin). There were eight inocula- 
tions made on the vines and seven on fruits, but no visible 
effects were produced on either, yet, a few of the dead slivers 
(resulting from the tangential cut preceding inoculation) on 
the vines developed typical perithecia with spores. No pyc- 
nidia could be found. 
5. Lagenaria vulgaris (Sugar Trough Gourd). Forty inocu- 
lations were made on fruits and vines, with no apparent affect 
upon the host, but a few fully developed perithecia with spores 
were found on two dead vine-slivers. 
