2o2 REPORT OF THE BOTANICAL DEPARTMENT OF THE 
ing wax and the others left uncovered. They were all effective 
except four of the covered ones on muskmelon. Both pycnidia 
and perithecia occurred on these inoculations. 
The stock cultures were retransferred to tubes of potato-agar. 
A few days later another, but smaller, set of inoculations was 
made. Only a few of the uncovered ones were effective and 
produced pycnidia. The cultures were found to be contami- 
nated with a bacillus, which after losing its motility developed 
great numbers of dumb-bell-shaped and globular, brownish in- 
volution forms. 
Ascosporic and pycnidiosporic inmoculations.—From freshly 
collected muskmelon vines (growing in the greenhouse) large 
quantities of pycnidiospores were obtained, suspended in steri- 
lized water, and transferred to the plants to be inoculated, by 
means of a sterile pipette. 
The vines from one muskmelon hill and one watermelon hill 
were inoculated. The uninjured nodes of each species were in- 
fected with spores. Every inoculated node was covered with 
wet, sterilized absorbent cotton. Equal numbers of similar in- 
oculations were made on both hosts, except that the nodes were 
punctured with a sterile forceps before the spores were trans- 
ferred to them. 
The cotton on all nodes was resaturated with sterile water 
one and a half hours after inoculation. The following morn- 
ing, fifteen hours after inoculation, sterile water was again 
applied. All but three of the cotton wads were still moist. 
Six hours later more water was added. 
Only one of the-forty inoculations became injurious and pro- 
duced fruits of the fungus: on a punctured node of muskmelon. 
Equal numbers of similar inoculations were made on other 
plants of the same hosts, using ascospores. The cotton was 
remoistened but once: three hours after inoculation. Neither 
the injured nor uninjured nodes were parasitized. The spore 
inoculations of 1907. (p. 278) no doubt were effective because 
they were put in direct contact with the inner host tissues and 
were provided with both moisture and nutritive matter under 
the protecting wax. ; 
