New York AGRICULTURAL EXPERIMENT STATION. 375 
The cultures—Hanging drop cultures were made in Van Tieg- 
hem cells 10 to 12 mm. inside diameter and about 10 mm. high 
sealed with a mixture of wax and vaseline. The pollen was 
placed on a clean cover glass, a drop of the culture liquid was 
applied and some of the same was put into the bottom of the 
cell so that the liquid in the cell would be of the same composi- 
tion as that in the drop. The cover glass was then put into its 
place and sealed. 
POLLEN CULTURES IN MIXTURE A. 
A spray mixture which for convenience of reference is here 
“ealled “A” was made from the ordinary commercial materials. 
It consisted of Bordeaux mixture prepared as is usually done for 
spraying orchards. Fhis was made according to the 1-to-1l 
formula An arsenical poison, the green arsenite of copper, was 
then added at the rate of about 1 lb. to 150 gallons, the amount 
commonly recommended for spraying orchards. Mr. W. H. 
’ Andrews very kindly analyzed this green arsenite and found 57.5 
per ct. arsenious oxide and 25.2 per ct. copper oxide. This shows 
that as an insecticide it is equivalent to good Paris green. 
An anther from a Ben Davis apple blossom was selected May 
18 in the way already described. From the pollen which it fur- 
nished a series of cultures was made as recorded below. This is 
here called Series I. 
SErizes I. 
Number of pollen 
Culture é grains in the 
No. Medium. culture. 
9 2 per ct. aqueous cane sugar solution, 99 parts; mix- 
UI PO-DALTL og se cssise be eeicatauwace sigeevasasee vas 250 to 300 
10 2 per ct. aqueous cane sugar solution, 9914 parts; 
PERE Oe TILT 5s cc. 0 oleae prende eh be we ene aia wis 125 to 150 
11 2 per ct. aqueous cane sugar solder. 99.98 parts; 
Te ALC. . hc ote ce ceed. wa tia aelsdme adele -- 125 to 150 
12 2 per ct. aqueous cane sugar solution, 99.99 parts; 
TEE TIAT oc oc aces ce on cidswaupewicceenes « 100 to 125 
As soon as these cultures were made, 5.45 p. m., they were 
put into a room having a nearly constant temperature of about 
*This formula requires 1 lb. of copper sulphate, and the necessary 
amount of lime, in making 11 gallons of the mixture. It is equivalent to 
10.9 grams of copper sulphate for 1 litre of the mixture. 
