016 REPORT OF THE HORTICULTURAL DEPARTMENT OF THB 
70° F. or slightly more. At 9.30 a. m. of the following day per- 
haps half of the, grains in No. 9 had germinated but they showed 
decidedly the retarding effect of the poisons. In No. 10 the ger- 
mination and growth were no better than in No. 9, really not 
quite so good. In No. 11 the grains germinated freely; nearly 
or quite all of them had germinated and the tubes had made free 
growth. In No, 12 germination and growth had progressed as 
freely as in No. 11. 
At 5.20 p. m. of the same day, about 24 hours acter the cul- 
tures had been started, these notes were made. ! 
No. 9. Pollen tubes vary from 1.96 to 18.48” long; average — 
length perhaps 10. Many tubes which were apparently in per- 
fect condition this morning have now broken down and 
become disintegrated. Many of the pollen grains have not yet 
germinated. 
No. 10. Pollen tubes vary from 1.68, to 14» long. They 
average perhaps 7. Some of the pollen tubes have pag id dis- 
integrated like those noted in No. 9. 
No. 11. The pollen tubes have made good eLow They 
vary in length from 10.674 to 87.36» and average perhaps from 
60 to 75. Occasionally tubes are seen which are abnormally 
swollen and give some appearance of breaking down. 
No. 12. The pollen tubes have made good growth. The tubes 
vary in length from 8.44 to 75.64 or more. No disintegration of 
pollen tubes was noticed. 
At 5 p. m., May 22, four days after these cultures were made, 
the following notes were taken and the cultures were then 
discarded. | 
No. 9. It is estimated that from 380 per ct. to 50 per ct. of the 
pollen grains in this culture have germinated. One of the 
longest tubes measured 51.96, but such length was quite excep- 
tional. Most of the tubes were less than one-half or even one- 
third of that length; many were no longer than from 5,4 to 10 
The tubes are apt to be abnormally eurved or twisted and the - 
growth is not vigorous. Indeed there is an appearance of dis- 
integration of some of the tubes. 

