New YorK AGRICULTURAL EXPERIMENT STATION. 5 Gr 
No. 10.. It is estimated that no more than 25 per ct. have 
germinated. The growth is correspondingly less than in No. 9. 
One of the longest tubes measured 27,. The tubes probably do 
not average half that length. Oftentimes they are abnormally 
bent and in many cases disintegration is seen. 
No. 11. This culture shows a network of long tubes. One 
which was measured was 98.4 long, but it cannot be said that 
this is one of the longest of them. Only in a few cases can tubes 
be traced through their entire length because they are so much 
tangled, and a close estimate of their average length cannot be 
made. It may be said, however, that they have made abundant 
growth. 
No. 12. Pollen tubes are tangled so much that only in few 
instances can they be traced throughout their entire length. 
One measured 52.56u, but others were certainly longer. The 
average length is estimated as considerably less than in No. 11. 
Only occasional indications of disintegration were seen. 
POLLEN CULTURES IN MIXTURE B. 
The spray mixture which is here designated “‘ mixture B ” was 
simply a Bordeaux mixture made as for “mixture A,” but it 
did not have any arsenical poison added to it. A single anther 
from a Ben Davis apple blossom was selected Ilay 17 in the way 
previously described, p. 374, to furnish pollen for a series of 
tests in which the growth of pollen in solution of sugar in water 
might be compared wit. its'growth in a similar sug*,r solution 
combined with various proportions of the Bordeaux mixture B. 
The following cultures were made at 5 p. m., May 17. They 
were put into a room in which by means of an automatic appara- 
tus the temperature was kept. nearly constant at about 70° F. 
PO 2 Ks 
Series II, 
Number of pollen 
Culture grains in the 
No. Medium. culture. 
4 2 per ct. aqueous solution cane SUgar.........00- Several hundred 
5 5 per ct. aqueous solution cane sugar............ Not recorded 
6 10 per ct. aqueous solution cane sugar.......... About 250 
7 5S per ct. aqueous solution cane sugar, 95 parts; 
BGrdeCamx (iis Citar emeeOtes. sc beeen sees About 300 
8 2 per ct. aqueous solution cane sugar, 98 parts; 
Borden umeMixture Pes 2e parte... «cence ccc planes Several hundred 
