378 Report oF THE HORTICULTURAL DEPARTMENT OF THE 
May 18, at 10.30 a. m., the following notes were madé on these 
cultures. 
No. 4. Nearly every grain has germinated and gent out a 
vigorous pollen tube. The tubes vary in length from about 15 
to 130” or more. The growth is so much tangled that it is 
impossible to measure each tube. The average length may be 
estimated at about 80u. | 
No. 5. The percentage of germination is almost as high as in 
No. 4. The tubes vary in length from 9.5, to 175,» or more. 
The growth is matted as noted under No. 4 so that it is impos- 
sible to get the exact measurement of each tube. 
No. 6. It appears that 50 per ct. of the pollen grains have 
germinated, but the average length of the tubes is much shorter 
than either those of No. 4 or No. 5. The tubes vary from about 
7vto about 86 or possibly more. 
No. 7. None has germinated. 
No. 8. None has germinated. 
At 6 p. m. the same date neither 7 nor 8 had yet shown any 
germination. The condition of Nos. 4, 5 and 6 was relatively ~ 
about the same as when the last observation was made. This 
series of cultures was discarded at 4:40 p. m., May 22. At that 
time 50 per ct. of the pollen grains had germinated in No. 6, the 
10 per ct. sugar solution. In No. 7, which contained 5 per ct. 
Bordeaux mixture B and 5 per ct. sugar solution no germination 
was found. But in No. 8, which contained 2 per ct. Bordeaux 
mixture B and 2 per ct. sugar solution a single pollen tube was 
found. This was 8.364 long. One other pollen grain had the 
appearance of having sent out a tube of about the same length 
which had disintegrated before it was observed. The other 
cultures, 4 and 5, so far as could be determined retained about 
the same relative rank as to growth as was noted before, but 
the growth was too abundant and the tubes too much matted 
together to make definite conclusion on this point. It was 
noticed, however, that some pollen grains in 5 had not yet ger- | 
minated. 
