New YorK AGRICULTURAL EXPERIMENT STATION. 379 
POLLEN CULTURES IN MIXTURB OC. 
The mixture which for convenience of reference is here called 
C consisted of a Bordeaux mixture made wholly of chemically 
pure ingredients combined with a solution of cane sugar in 
water. The formula used was 1 gram of copper sulphate and # 
gram of lime to make 100 ce. Bordeaux mixture.t After the cop- 
' per sulphate solution and lime had been combined and diluted 
to 96 cc. with distilled water 4 cc. of 50 per ct. aqueous cane 
sugar solution were added to bring the whole mixture up to 
100 ce. Work with mixture C did not begin until after apples 
were out of bloom and so the results given with it were obtained 
with other kinds of pollen than that used for the cultures in 
mixtures A and B. 
Van Tieghem cell cultures which were made in the way 
previously described, p. 375, were prepared June 28. In each 
one was put some blackberry pollen from the same anther. The 
following statement shows the medium used for each culture. 
SERIEs III. 
Culture ~ Number of pollen 
No. Medium. grains in culture. 
15 Mixture C, 100 parts.......... alse Gah epiomts Chesie 128 
16 Mixture C, 2 parts; 2 per ct. aqueous solution of 
PMN ie i. THATUR: ss, ce sletenit hd eardaiclote we tle < 115 
17 Mixture C, 1 part; 2 per ct. aqueous solution of 
Seen SUOMI! OO) DALLG. 50 <i cclce cara eek wee ee eee 8 108 
18 Mixture C, % part; 2 per ct. aqueous solution of 
IIOP AT. N04” DALUS sc edus ¢e4cs vs qe0 s¥neess 3 64 
19 Mixture C, *% part; 2 per ct. aqueous solution of 
Damme sures 09 £5 DATES ois ware dn fbb cee erste inleiege's oie 43 
20 Copper arsenite and lime, 1 gram to 1% liters; in 
2 per ct. aqueous solution? of cane sugar........ About 12 
21 Lime, 1 gram to 1.25 liters of a 2 per ct. aqueous 
solution® of cane sugar.............-. OWS iar asks 69 
22 Cane sugar, 2 per ct. aqueous solution..... Peale was 107 
1This is the same as 10 grams copper sulphate to make 1 liter of Bor- 
deaux mixture which formula is nearly equivalent to the 1-to-11 formula. 
See p. 375. 
2This is at the rate of about 1 lb. to 150 gallons. 
’The copper arsenite, CuHAsO3, was used at the rate of 1 gram to 1.25 
liters. This is approximately the strength at which either this or Paris 
green is used in orchard spraying; i.e., about 11b. to 150 gallons. Lime 
was added at the same rate as the copper arsenite; i. e., 1 gram to 1.25 
liters, and cane sugar solution at a rate to make the whole a 2 per ct. 
solution of cane sugar. 
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