New YorkK AGRICULTURAL EXPERIMENT STATION. 881 
room having a temperature of 70° F. On July 2, after being 
kept three days many more germinations were found in No. 21 
but none in No. 30. 
Cultures of pollen of the Virginia Creeper, Ampelopsis quin- 
quefolia, all from the same anther, were made at 11:30 a. M., 
July 25, 1900, as listed below. 
SERIES VY. 
Number Per ct. 
of pollen Number of ger- 
Culture grains in germi- mina- 
No. Medium. culture. nated. tion. 
85 2 per ct. aqueous cane sugar solution, 
2 parts; water, 98 parts.......seeeees 88 66 15 
36 Lime, 1 gram to 125 ce. of a 2 per ct. 
aqueous solution of cane sugar..... 144 0 0 
37 Copper arsenite and lime’ and a 2 per 
é ct, aqueous solution cane sugar..... : 230 0. 0 
58 Mixture C, ss part; aqueous solution of 
Pee BUGAI OOSe DATUS. ; osisa ce cides 203 136 67 
39 Mixture C, % part; aqueous solution of . 
eane sugar 9016: parish. cic oi eies wees 118 5 4 
40 Mixture C, 1 part; aqueous solution of 
WaMe SUS ATS Te DAEs ose ae cain seat atic tat ; 187 0 0 
41 Mixture C, 2 parts; aqueous solution of 
Calas SURAT SOB PALS y % wie oid peers vw anes - 122 0 0 
After six hours it was found that many pollen grains had ger- 
minated in Nos. 35 and 88. Five had also germinated in No. 39. 
There were no germinations in any of the other cultures. At 
§:30 a. M., July 26, after the cultures had been made 21 hours 
it was found that there were 66 germinations in No. 35, or 75 
per ct.; in No. 88 there were 136 or 67 per ct.; and in No. 39 there 
were 5, or 4 perct. In none of the other cultures was any ger- 
mination found. After being kept 24 hours longer no further 
germination was found and these cultures were discarded. 
Cultures of the pollen of Japan ivy, or Boston ivy, Ampelopsis 
tricuspidata, were made at 11:30 a. M., July 30, using the same 
media as in Nos. 87 to 41, and also one culture was made in a 
2 per ct. sugar solution in water alone. 
1Prepared as for culture No. 20, see p, 379. 
