116 REPORT OF THE BOTANIST OF THE 
were either wilted or dried up. For a short distance, both above 
and below the wax, the bark was dead and brown and the wood 
discolored. In every case the dead bark in the vicinity of the 
wax (both above and below it) was covered with Coniothyrium 
pycnidia containing mature spores. One cane, broken accident- 
ally, was very brittle at the point of inoculation as is the case with 
affected canes in nature. Although this experiment had no check 
the results indicated that the Coniothyriwm is parasitic. 
Haperiment No. 2.— Date of inoculation, July 18, 1900. Four 
new unbranched canes of black raspberry (variety, Mohler) in- 
oculated, as in Experiment No. 1, by abrading the bark, applying 
fungus and winding with grafting wax. Two other canes of the 
same variety but considerably branched were inoculated in a 
freshly cut stub, such as is commonly left in “ heading back ” the 
canes. The stub was covered with grafting wax. The fungus 
culture used was the same as that used in Experiment No. 1. | 
There were no checks. 
On October 1, 1900, two of the unbranched canes were dead 
and the other two had prominent scars at the point of inoculation. 
Whether these two canes were killed by Coniothyrium is un- 
certain. One showed an abundance of Coniothyrium, both above 
and below the wax, but on the other there was no sign of 
Coniothyrium. At this date the two branched canes inoculated 
in the stub were beginning to show the effects of inoculation. On 
one, the uppermost branch was already involved. 
The last observations were made July 8, 1901, at which date 
one of the two remaining unbranched canes was nearly dead 
while the other was alive and apparently little harmed by inocu- 
lation. The dying cane showed an abundance of Coniothyrium 
pycnidia scattered along one side for a distance of several inches 
below the point of inoculation. One of the two branched canes 
inoculated in the stub was dying in the upper portion although 
it bore a fair crop of fruit. Two of the upper branches were 
dead. The fungus had worked down the cane about five inches 
from the point of inoculation and pycnidia were plentiful. The 
condition of the other branched cane was unchanged since the 
preceding October. It was uninjured. 
