New YorK AGRICULTURAL EXPERIMENT STATION. 121 
applied and the cane wound with grafting wax. There were 
also five checks to this part of the experiment. 
Winally, five other canes were inoculated by puncturing with 
an awl and applying fungus and distilled water, but the canes 
were not wound with wax. 
The fungus used throughout this experiment consisted of a 
mixture of two cultures, one of which was twelve days old and 
the other fifty days. It was descended from the fungus used in 
Experiments 3-7 inclusive, and was originally isolated May 1, 
1901. 
On August 8, 1902, all 25 canes in the experiment were cut 
and each one carefully examined. Of the five canes inoculated 
by abrasion four were dead from the wax up and covered with 
Coniothyrium pycnidia around the wax, both above and below 
it. The bark was dead and the wood discolored from two to 
three inches below the point of inoculation. The fifth cane was 
alive, but at the point of inoculation the wood was discolored 
and bore Coniothyriwm pycnidia. Of the five checks belonging 
to this part of the experiment four were alive and green with 
no discoloration of the bark or wood anywhere, and with no 
sign of Coniothyriwm. The remaining check was still alive, 
but had a pronounced discoloration of the bark and wood each 
side of the wax and an abundance of Coniothyrium was present. 
In some way it had become infected. 
Of the five canes inoculated in a puncture covered with wax 
every one showed an abundance of Coniothyrium pycnidia in the 
vicinity of the wax. Four were dead and broken at the point 
ef inoculation, while the fifth one, although still alive, had the 
bark dead and wood discolored one-half way around the cane 
for a distance of four inches above and two inches below the 
wax and thickly covered with Coniothyriuwm pycnidia. This 
eane was considerably larger than the other four, which prob- 
ably explains its greater resistance to the disease. The five 
ehecks belonging to this part of the experiment were all alive 
and green with no discoloration of the bark or wood around the 
puncture in any case and none of the canes were broken. 
Of the five canes inoculated in punctures, which were not 
eovered with wax, every one showed an abundance of Coniothy- 
