154 Report oF tHe BorTaNist OF THE 
Pure cultures of the fungus obtained from apples and from 
cordwood were used in all of the inoculations. The same char- 
acteristic rot was produced whether inoculated with a culture 
made from a diseased apple or from the fungus as it was found 
growing as a saprophyte on cordwood. 
The method of inoculation was as follows: The fruits were 
carefully selected and only sound, perfect specimens taken. They 
were sterilized by immersing in a 1-1000 solution of corrosive 
sublimate. This was washed off with distilled water and the 
surplus water was removed with a piece of sterilized cotton. In 
each case two fruits of the same variety were placed in a large, 
moist chamber that had been washed out with a 1-1000 solution 
of corrosive sublimate. The epidermis of each fruit was punc- 
tured with a sterilized needle. With another sterilized needle 
some of the fungus growing pure on slices of sugar beet, potato 
agar or apple agar was placed in the puncture of one fruit, the 
other being reserved for the check. (Plates XVIII and XIX.) 
