156 REPORT OF THE BOTANIST OF THE 
Inoculations conducted under the same sterile conditions as 
above described were made to determine if the fungus could pen- 
etrate the unbroken epidermis and cause the rot. Spores from 
a pure culture of the fungus were placed on one fruit without 
puncturing or breaking the epidermis, and on the other fruit 
inoculation was made by puncture, as previously described. The 
rot did not develop where the spores were placed on the unbroken 
epidermis, except in one case, and that was where a very tender- 
skinned variety was used. On all of the fruits inoculated by 
puncture the characteristic rot developed. 
Taste II.— INocuLATIONS MADE ON SouND EPIDERMIS AND 
THROUGH A PUNCTURE. 







ial 2 o§ 
cs VARIETY, E °¢ Condition. 
ger | fe 2'3 
A° sl as 
Oct. 11 | Raymond de Montlaur*....) Sound.....) Oct. 22 | Fruit sound. 
11 | Raymond de Montlaur*....| Punctured. 22 | Rot developed. 
11 | Therese Appert*.......... Sound ....| 22 | Fruit sound. 
11 | Therese Appert*...... *....| Punctured.| 22 | Rot developed. 
Il | ‘Golden Sweetas.ino/. PSS :; Sound.....| 22 | Fruit sound. 
11 | Golden Sweet ............ Punctured. 22 | Rot developed. 
1 |} Coz Pomtnast) <i oh 28.4 Sound..... 22 | Fruit sound. 
El Cee. Pomons... aa eee Punctured . 22 | Rot developed. 
11 | Small Admirable..7....... Sound... .. 22 | Rot developed. 
11 | Small Admirable.....,.... Punctured. 22 Rot developed. 
Abr) Misther 2, arate rw a Sound..... 22 | Fruit sound. 
LY's) Mothertic.?. egies) ASS Punctured. 22 | Rot developed. 



* These two are pears, the others are apples. 
The results of these inoculations indicate that the fungus is 
more properly classified as a wound parasite rather than as a 
true parasite. 
[ixperiments were made to determine whether the disease would 
work more vigorously when inoculation was made upon a seab 
spot or by puncture through healthy epidermis. As in the other 
experiments, two fruits of each variety were used — one on which 
there was a fair-sized scab spot, the other with the epidermis all 
sound. Inoculation was made by placing the spores from a pure 
culture of the fungus upon the scab spot of one fruit and by 
puncture in the usual way on the sound fruit. 
