220 REPORT OF THE CHEMIST OF THE 
figures given are on the basis of ash-free substance. The first 
five samples were prepared by dialysis and precipitation by 
alcohol in neutral or slightly alkaline solution, except sample 3, 
in which case the solution was probably slightly acid. The other 
samples were prepared by the first method described above. In 
samples 6, 7 and 8 the amount of material obtained was not suf- 
ficient for making all the determinations. 
TABLE J.—ANALYSIS OF PREPARATIONS OF PARANUCLEIN OBTAINED 
FROM DIFFERENT CHEESES. 






Per cto) Perici) Rerict: | Perrct. | Persct®) Per et.;\) Perve. 
1. Cheese bought in open 







market, 3 months old.} 48.511. 6.81) 14.14) 3.19) 2.50) 24.95) 31.53 
2. Cheese bought in open / 
market, 3 months old.) 48.46} 5.67) 13.25) 2.00) 2.23) 28.39 23.65 
3. Cheese 44 months old..; 52.01} 6.98) 14.17; 1.65) 1.41) 23.78 1.44 
4. Cheese 7 months old, in- a 
LORLOT, 3674/4 epee aye | 50.60) 7.00) 13.65) 1.00) 1.33) 26.32) 16.64 
5. Same, 7 months old, ex- I] 
teriok sO FUE, tH 50.56) 7.70) 14.36; 0.86) 1.52) 25.00) 20.22 
6. Cheese 1 month old....| 46.72} 6.86] 15.91)...... be wea tas te ies. Sane 
7. Same, 2 months old... .| 47.59]. 6.90) 12.36). ..... ae the one ee 
8. Same, 3 months old... .| 48.82) 7.43) 12.98)...... | evics-p ‘yall ca Be ae 
9. Same, 4 months old....| 50.56} 6.73) 12.96) 0.83). 1.13) 27.7 3.10 
10. Same, 5 months old... | 53.12} 6.51) 13.68) 0.89 0.98) 24.82) 4.42 


An examination of the results indicates that we probably did 
not succeed in freeing the substance from impurities. The few 
analyses of nuclein on record show considerable divergence. In 
comparison with Chittenden’s casein-dyspeptone, the content of 
nitrogen, hydrogen and carbon is lower in our substance, while 
the sulphur is considerably higher. Chittenden regarded the 
phosphorus as consisting entirely of calcium phosphate, but 
Jackson has shown that paranuclein yields over 2 per ct. of 
organic phosphorus, if care is taken to prevent calcium phos- 
phate from being precipitated with the substance and to avoid 
the formation of inorganic phosphates during ignition. We 
shall give our aitention further to methods of separating 
paranuclein from cheese in the hope of being able to prepare it 
free from impurities and studying its decomposition products. 
