New YorkK AGRICULTURAL EXPERIMENT STATION. 223 
LYASTINE. 
In cheese 4 1-2 months old. —To Solution B, obtained as de- 
scribed above, we added just enough barium nitrate to remove all 
sulphuric acid, filtered and concentrated the filtrate to small bulk. 
Silver nitrate was carefully added until the presence of an excess 
was shown by the usuai test and then a few drops of dilute nitric 
acid were added. Alcohol was next added and a precipitate be- 
gan to form, when the addition of alcohol was continued until its 
volume equalled the volume of the solution. Then a small amount 
of ether was added, when a crystalline precipitate was formed. 
This was allowed to stand 24 hours and then filtered, washed with 
ether and dried over sulphuric acid, yielding a product weighing 
2.1 grams. The filtrate was used for separating histidine. This 
product behaved like Siegfried’s lysatine and analysis shows its 
similarity to that body. 
().202 gram substance gave 0.0565 gram Ag. 
0.214 gram substance gave 74.5 cc. N at 22° C. and 746 mm. 
Percentages: Calculated for lyastine silver nitrate, Found 
(C, Ais N,0, HNO, Ag NO,) 
Ag 27.51 27.9% 
N 17.90 17.89 
A portion of the lyastine silver nitrate, obtaining as described 
above, was treated with hydrochloric acid and evaporated nearly 
to dryness, this operation being repeated several times to remove 
the greater part of the nitric acid. Platinum chloride was then 
added and the solution evaporated nearly to dryness, when, on 
addition of alcohol, the platinum salt of lyastine separated. 
This was filtered, washed with alcohol and dried over sulphuric 
acid in vacuo. Analysis gave the following results: 
0.2414 gram substance gave 0.0835 gram Pt. 
Percentages: Calculated for lysatine platinum chloride, Found 
fOr Oe tls Peony 
gs | 34.28 34.58 
HISTIDINE. 
In cheese 4 1-2 months old.— The filtrate obtained from the 
precipitate of lysatine, as described above, was carefully evapo- 
rated on the water-bath to remove ether and alcohol and then 
