934 ttepokt Of THE CHEMistT OF THE 
treated with barium hydroxide until one drop of the clear super- 
natant liquid, added to an ammoniacal solution of silver nitrate, 
gave no precipitate, thus showing the complete precipitation of 
histidine. This precipitate was filtered, washed with water and 
then suspended in water and treated with hydrogen sulphide to 
remove silver. After the silver sulphide was filtered, the filtrate 
was evaporated to dryness, this residue extracted with dilute 
Silver nitrale, containing a few drops of nitric acid and the ex- 
tract precipitated with ammonia, filtered, and washed with 
water. This precipitate was next suspended in water, decom- 
posed by hydrochloric acid, the silver chloride filtered and 
washed and the filtrate concentrated and allowed to stand 
several days, when crystals began to separate. After standing 
a few days longer, the liquid was filled with crystals. The crys- 
talline mass was washed with ether and dried over sulphuric 
acid mm vacuo, yielding 4.1 grams histidine. 
0.374 gram substance gave 0.1156 gram Cl. 
(0.2918 gram substance gave 0.532 gram N. 
Percentages: Calculated for histidine hydrochloride, Found 
(C,H,N,0,2HCl) 
Cl 31.11 30.98 
N 18.42 18.28 
ARGININE. 
In cheese 4 1-2 months old.— The filtrate from the histidine 
precipitate was saturated with bariuin hydroxide, the precipitate 
filtered, washed with solution of barium hydroxide, suspended in 
water acidulated with sulphuric acid, and the silver removed by 
hydrogen sulphide. The entire filtrate contained only 0.085 gram 
nitrogen, equivalent to 0.255 gram arginine. The amount was 
too small to obtain as a crystalline product, and we are led to 
conclude that arginine does not exist in appreciable quantities in 
cheese of this age. Winterstein and Thény! have also reported 
a Similar result in case of emmenthaler cheese, the age of which, 
however, they do not state. 
10Zeit. f. Physiol. Chem., 36: 28 (1902). 
