226 REpoRT OF THE CHEMIST OF THE 
filtered, washed with alcohol, recrystallized three times from 
water, finally washed with ether and dried over sulphuric acid 
yielding 44.1 grams (Fraction No. 3). 
The last filtrate was further concentrated by evaporation and 
yielded an additional crop of crystals, weighing 0.2 gram (Frac- 
tion No. 4). 
The crystals contained in Fractions 3 and 4 behaved like lysine 
and showed, on analysis, a composition corresponding to this 
compound. 
Percentages: Calculated for lysine picrate. Found 
(C,H,,N,0, C, H, (NO,) 30 H) 
N 18.66 19.17% 
C 38.40 38.18 
H 4.53 4,21 
On converting this salt to a chloride and estimating the chlo- 
rine, the following results were obtained: 
0.277 gram substance gave 0.3616 AgCl. 
Percentages: Calculated for lysine hydrochloride. Found 
(C,H,,N,0O, 2HCl) 
Cl 32.39 32,24 
In cheese 4 1-2 months old.—We obtained 5.4 grams of lysine. 
A portion recrystallized several times from water gave 18.81 N, 
the calculated amount being 18.66 N. 
TETRAMETHYLENEDIAMINE (PUTRESCINE). 
In cheese 15 months old.— Fraction No. 1 of crystals, obtained 
as described above, resembled tetramethylenediamine in its solu- 
bilities and an analysis showed its identity in composition with 
this compound. 
Percentages : 
Calculated for tetramethylenediamine picrate. Found 
(C,H,.N, 20,H,(NO,) 380 H) 
N 20.51 20.34 
H 3.29 3.48 
C 35.16 35.40 
On converting this compound to a chloride, the following 
amount of chlorine was obtained: 
0.206 gram substance gave 0.3865 gram AgCl. 
