374 REPORT OF THE HORTICULTURAL DEPARTMENT OF THB 
In addition to the tests above described a few others were 
made, using more careful methods to insure both perfect castra- 
tion without either injury to the pistil or self-pollination of it, 
and also an abundant application of the pollen used in cross- 
pollination. On June 28 unopened buds of Black Eagle were 
covered with manilla bags to prevent the access of foreign 
pollen. On June 29 they had begun to open. The whole cluster, 
without being removed from the bag, was cut from the vine and 
taken to the laboratory, where the anthers were removed, put 
into a wide, short test tube and shaken till the bottom of the 
tube was well covered with pollen. A cluster of Bailey, in 
which the buds were nearly ready to open, was then selected. 
Part of the buds were cut out and the rest, 24 in number, were 
very carefully castrated by means of pliers and scalpel. Both 
the tools and the fingers were repeatedly dipped into strong 
alcohol during the operation, to prevent accidental self-pollina- 
tion. Any bud in which any dehiscence of the anther appeared 
was at once discarded. After castration the cluster was 
pushed into the tube as far as the tube would contain it and 
the naked stigmas brushed against the sides and bottom, which 
were covered with the Black Eagle pollen. Additional pollen 
was then applied to each pistil by means of a camel’s-hair brush. 
Finally the cluster was inclosed in a manilla paper bag to pre- 
vent the access of other pollen. In a corresponding way other 
crosses were made. The name of the parents and the number - 
of pistils treated are shown in the following list: 
