New York AGRICULTURAL EXPERIMENT STATION. 393 
CULTURES. 
In 1 per ct. sugar solution.— The results from the first four 
kinds of pollen tested were uncertain. ‘This was due to using. 
a sugar solution which was too weak for this kind of pollen to 
make a good growth (1 per ct.). The only difference shown in 
this pollen was in the budding, which is the first stage in pollen 
germination. See Fig. 1. None germinated. The Clinton and 
Janesville both showed buds on from 5 to 10 per ct. of the 
grains, but the Clevener and Marion showed at the end of four 
days no change from their condition at time when placed in 
solution. 
In 2 1-2 per ct. sugar solution On June 18 pollen grains of 
Elvibach, Berckmans, Aminia and Agawam were placed in hang- 
ing drops of 2144 per ct. sugar solution. They were examined 
for three successive days thereafter and the number of germina- 
tions noted. The Elvibach and Aminia pollen did not germi- 
nate or even bud. About 4 per ct. of the Berckmans germinated 
and about 10 per ct. of the Agawam. 
In 5 per ct. dextrose solution.—On June 21 pollen was prepared 
as before, using a 5 per ct. solution of dextrose in place of the 
previous medium. The varieties from which pollen was taken 
in this instance were Grein Golden, Missouri Reisling, Barry, 
Rogers No. 32, Wyoming and Lucile. Notes were taken on these 
cultures on June 23, at which time they were in the height of 
their growth, none germinating after that date. At this time 
the pollen grains of the Grein Golden, Barry and Wyoming had 
not changed in any way from their condition when they were 
first placed in the solution. About 12 per ct. of the Missouri 
Reisling germinated, 20 per ct. of Rogers No. 32 and 10 per ct. 
of the Lucile. 
In 10 per ct. dextrose solution.—Pollen of these same varieties 
was placed at this latter date in 10 per ct. dextrose solution with 
the following results: Out of an estimated 200 erains of pollen 
of Grein Golden, one made a very weak growth; Barry and 
Wyoming pollen grains not changed in any way; Missouri Reis- 
ling, about 15 per ct. germinated; Rogers No. 32, about 50 per ct. 
(especially strong); Lucile was accidentally destroyed. 
