New York AGRICULTURAL EXPERIMENT STATION. 335 
bark, by taking small particles from the inner bark with sterile 
instruments. Two forms of fungi appeared in these cultures 
more or less constantly, which led to their being separated and 
transferred to sterilized bean stems in test tubes. Here they 
grew luxuriantly and soon produced fruit, the one form produc- 
ing the familiar dark colored spores which were not at that time 
identified, while in the other the sporophores of Schizophyllum 
commune Fr. were formed. 
Inoculations were made with the cultures on June 22 on seed- 
ing apple trees in the nursery row as follows: Three trees were 
inoculated with material from cultures of the dark spored fungus, 
three trees with material from cultures of Schizophyllum com- 
mune, and three trees were punctured but not inoculated to serve 
as checks. The inoculations were made by cutting a small open- 
ing in the bark with a sterilized knife and inserting a small 
amount of the material from the bean stem cultures between the 
bark and wood. All of the punctures were covered with filter 
paper which was kept moist for about thirty-six hours. On the 
same date two inoculations with each of the two cultures, together 
with check wounds, were made in the larger limbs of a mature 
apple tree. These inoculations were not moistened or protected 
in any way. In two weeks’ time there was an area of discolored 
bark around each place of inoculation where the unknown fungus 
_had been inserted. The other inoculations as well as the checks 
showed no signs of growth and the wounds soon healed. 
As soon as it was known that the one fungus could penetrate 
living bark under certain conditions more inoculations were made. 
July 6, six young seedling apple trees in the nursery row and 
three limbs of a large apple tree were inoculated with the dark 
spored fungus, six seedling nursery trees and three limbs of 
a larger tree with Schizophyllum commune, while three seedling 
nursery trees and three limbs of a large tree were punctured but 
not inoculated to serve as checks. The inoculations made in the 
‘seedling trees were all protected with filter paper as before, but 
