After 2 months, no significant worm mortality was found. In the second 
test, worms were similarly exposed to lindane at rates of 32, 64, and 
128 1b/A. Smaller, capped containers were used to prevent worm escape 
at the high dosages. The LD5p at 96 hours was computed at 60 1b/6-in A. 
Lipa (1958) used a 12% lindane dust in treating a field used 
to grow potatoes and sugar beets. Plots were treated in May at 1, 2, 
3, and 6 1b/A of toxicant; 18 months later the plots treated at 1, 2, 
and 3 1b/A-contained an average of more than twice as many earthworms 
as untreated plots. However, plots treated at 6 1b/A averaged the same 
number of worms as untreated plots. Data on worm species and the extent 
of sampling were not given. 
Captan 
Captan was tested on Eisenia foetida (sp.?) by Martin and 
Wiggans (1959). Worms were immersed for 2 hours in solutions containing 
0.1, 1, 10, 100, and 1,000 ppm and then kept in peat moss for an 
additional 24 hours, There was little worm mortality as a result of 
exposure at 10 ppm or below. All worms died from exposure at 100 or 
1,000 ppm. No attempt was made to transfer these data to field applica- 
tion rates of the chemical. 
Chlordane 
Schread (1952) tested chlordane for control of Pheretima 
hupeiensis. He used wettable powder as a dust, mixed with water, or 
mixed with fertilizer. Six months and a year after an application of 
43 1b/A, worm activity had ceased. At 64 1b/A, heavy mortality occurred 
after 2 weeks, and infestations were absent a year later. In another 
series of tests, 48% water emulsions produced heavy mortality at 20 and 
40 1b/A. At 40 1b/A, reinfestations did not occur in the first year. 
The emulsion acted more quickly than the wettable powder. 
Polivka (1953) used technical chlordane at rates of 5, 10, and 
20 1b/A on a golf course in Ohio. No reduction in earthworm populations 
had occurred within 1 month of an April treatment. After 1 year, how- 
ever, all treated plots had significantly lower earthworm populations 
than untreated plots. In another test, he used chlordane at 10 1b/A in 
emulsion form with 1,000, 100, and 9 gallons of water. Earthworm popula- 
tions were checked each week for 5 weeks after the April treatment, but 
no significant change was observed, possibly because of the masking 
effect of the normal population change among earthworms in early summer. 
Polivka concluded that fall worm populations in one other series of 
plots treated with chlordane 5 and 6 years previously at 5, 15, and 
25 1b/A still were significantly lower than those on control plots. 
Here again, the extent of the sampling was not presented. 
