\ 
AND SPECIES HYBRIDS WITHIN THE GENUS SACCHARUM Lt 7 
somatic chromosomenumber only could be determined, as this 
cane has not flowered at Pasoeroean during the last two years. 
Black Cheribon. Stems rather thick, dark-violet, with a bluish wax- 
covering. Internodes cilindrical. „Leafcrown” broad, fanshaped, 
drooping. High cane- and sugarproduction. Flowers frequently, 
with large inflorescence. Male entirely sterile, the anthers remai- 
ning closed and frequently being dry and shrivelled. Black Cheri- 
boniscultivatedin many sugar-growing countries and wassent from. 
Tucuman in the Argentine to the experiment-station at Pasoe- 
roean under the names: no. 6 Morada Tucwman, No. 55 of Bresil 
and no. 58 of Bresil; of Louisiana this kind was received as Lowisi- 
ana purple, while it is also much cultivated in Egypt. 
The fixation of the material always took place in the morning, usual- 
ly between 7 and 9. As fixation of the flowers is impossible in the field, 
promising canes had always to be transported to the laboratory. For this 
purpose they were cut close to the ground, placed in a bamboo-lid filled 
with water and as rapidly as possible brought into the laboratory, thus 
practically excluding the possibility of loss of water by the inflorescence. 
In the laboratory the inflorescence was deprived of its protecting 
leaf-sheaths; subsequently some lateral axes were taken at various 
heights from it and put into glass tubes containing the fixing fluid, al- 
ways taking care that the fluid surpassed in volume many times that 
of the material to be fixed. Immediately after, the tubes were put under 
the air-pump, so that the air was removed from the chaff and from the 
tissues. 
The following fixing-agents were tried. 
1°. A mixture of three parts of absolute alcohol and one part of ace- 
tic acid after Carnoy 3). 
2°. A mixture of 6 parts of absolute alcohol, 3 parts of chloroform 
and one part of acetic acid after Carnoy 2). 
3°. A mixture of 2 grams of zinkchloride, 2 cM? of acetic acid and 100 
cM? of 80% alcohol after Juel, modified by STRASSBURGER. 
4°. Flemming’s fixing fluid in weak solution after CHAMBERLAIN, 9) 
7) E. STRASSBURGER. Das Botanische Praktikum. 5te Auflage. 1913 p. 71. 
2) — — ibid. p. 64 
8) — — ibid. p. 70. 
4) Cu. J. CHAMBERLAIN. Methods in Plant Histology. Third revised edition 1915. 
