126 A CYTOLOGICAL INVESTIGATION OF SOME SPECIES 
in shape, about isodiametric und rather different in size. Here also their 
bivalent nature is hard to observe. Still, much depends on the indivi- 
dual of glagah under observation. Some individuals show the gemini 
clearly apart, others close together, in some they are more exactly 
situated in one plane than in others. In nuclei, viewed from the side, 
(fig. 14) the gemini are more or less diamondshaped, theyare very short; 
the two chromosomes forming them can not bedistinguished. It will be 
seen however, that univalent chromosomes differ distinctly in shape 
from the bivalent ones. Moreover these are frequently not lying exactly 
in the acquatorial plate, which is always the case with the gemini of 
Saccharum spontaneum. Counts of gemini are absolutely impossible in 
nuclear plates viewed from the side because those situated in the higher 
plane cover the others too much. 

Fig. 14. 
Fig. 14. Metaphase of the heterotype-division in side view of Saccharum spon- 
taneum X 2300. 
Of four kinds of Saccharum spontaneum, showing distinct differen- 
ces in habit, as well as in breadth of the leaves, in thickness of the stem, 
in shape and hoariness of the eye, and in shape and hoariness of the 
glumae, the chromosomenumber was investigated. 
By far the most beautifull nuclear plates were shown by a form col- 
lected near the desa (village) Kepandjen near Malang; this form has 
very narrow leaves and thin stems. Fig. 15 represents a nuclear plate 
of this form, clearly showing the presence of 56 chromosomes. Of 26 
other nuclear plates, 24 gave doubtless 56 as the chromosome-number, 
