AND SPECIES HYBRIDS WITHIN THE GENUS SACCHARUM 127 
once it was doubtfull whether 55 or 56 chromosomes were present and 
once 57 were counted, but in this case the anaphase had probably be- 
gun already. | 
In the case of a strong, broad-leaved form, collected on the sugar- 
plantation Djatiroto, 12 times 56 was found, two times I hesitated 
between 55 and 56 and once between 56 and 57. 
In the case of Glagah alas Kletak IIT, a form from the Tenger-moun- 
tains, the chromosomes of which lie much closer to one another in the 
nuclear plates, while the diameter of the plate moreover is smaller, 
three times 56, twice 57 and once 55 was found, so that in this case also 
56 was probably the correct number. 

Fig. 15. 
Fig. 15. Metaphase of the heterotype division in Saccharum spontaneum (Gla- 
gah Kepandjen) viewed from one of the poles x 2300. 
Glagah alas Troeno of the Ardjoeno-mountain, of which the two dia- 
kinesis-figures pictured in fig. 12 and 13, show 56 chromosomes, revea- 
led 4 times in metaphase the presence of 56, twice of 55 chromosomes, 
while twice again I hesitated between 55 and 56. 
Doubtless therefore the haploid chromosome-number of Saccharum 
spontaneum is 56. 
In the next division stage — the anaphase — the chromosomes of 
the gemini have separated and proceed towards the poles. The number 
of anaphases is always rather small in the preparations, showing that 
the chromosomes reach the poles quickly. 
Very rarely chromosomes lagging behind are seen. Fig. 16 shows an 
anaphase seen from. the side. In this cell the longitudinal splitting of 
the chromosomes, usually preparing the homotype division already 
during the anaphase of the heterotype one, could not be observed. 
